ASTM E1470-1992(2006) 电泳迁移率法表征蛋白质特性的标准试验方法

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【英文标准名称】:StandardTestMethodforCharacterizationofProteinsbyElectrophoreticMobility
【原文标准名称】:电泳迁移率法表征蛋白质特性的标准试验方法
【标准号】:ASTME1470-1992(2006)
【标准状态】:现行
【国别】:
【发布日期】:1992
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E48.03
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:电泳;作标记;蛋白质
【英文主题词】:electrophoreticmobility;molecularweight;proteins
【摘要】:Theprimepurposeofthistestmethodistoprovidedataexpressedaseitherelectrophoreticmobilityorzetapotentialdistributionofproteinparticles.Bothsellersandpurchasersofproteinparticleswillfindthistestmethodusefultodetermineeithermobilityorzetapotentialdistributionsforproteinspecifications,manufacturingcontrol,anddevelopmentandresearch.1.1Thistestmethoddescribesaprocedurefordeterminingtheelectrophoreticmobilityofproteinsofmolecularweightgreaterthan10000Daltons.1.2ThistestmethodusesautomaticElectrophoreticLightScattering(ELS)principlestodeterminetheelectrophoreticmobility.1.3TheinstrumentsimultaneouslymeasurestheDopplershiftsofscatteredlightatfourdifferentanglestodeterminetheelectrophoreticmobilitydistributionofproteinparticles.Themobilityisexpressedasm-cm/V-s(micron-centimeter/volt-second).Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:C04
【国际标准分类号】:07_080
【页数】:3P.;A4
【正文语种】: